TB-500 (Thymosin Beta-4)
Also known as TB-500 Frag, TB500 Frag, TB-500 Fragment, TB4 Frag, TB-4 Fragment, Thymosin Beta-4 Fragment, Thymosin Beta 4 Fragment
Typical dose
2–5 mg
loading 2×/wk × 4–6 wks, then weekly · SubQ
Half-life
2-3 days
What is TB-500 (Thymosin Beta-4)?
Synthetic peptide of Thymosin Beta-4. Powerful healing and recovery peptide used for tendon, ligament, and muscle repair.
How TB-500 (Thymosin Beta-4) works
Upregulates actin production, supports cell migration and differentiation for tissue repair.
Benefits & uses
- Injury recovery
- Muscle repair
- Flexibility
- Angiogenesis
- Tissue regeneration
Common uses: Injury recovery, muscle repair, flexibility, angiogenesis, tissue regeneration.
TB-500 (Thymosin Beta-4) dosage
- Typical dose
- Typical: loading 2–5 mg 2×/week for 4–6 weeks, then 2–5 mg/week maintenance. SubQ.
- Protocol notes
- 2-5mg weekly, can be split into 2 injections
TB-500 (Thymosin Beta-4) half-life
2-3 days
TB-500 (Thymosin Beta-4) side effects
Mild fatigue first week, minor injection reactions.
- Mild fatigue first week
- Minor injection reactions
Warnings & monitoring
- Research use only
- Mild fatigue first week
- Minor injection reactions
Preparation & storage
- Preparation
- Mix a 5 mg vial with 2 mL bac water → 2,500 mcg/mL. A 2.5 mg dose = 1 mL = 100 units.
- Reconstitution
- Reconstitute a 5 mg vial with 2 mL BAC water → 2.5 mg/mL. A 2.5 mg dose = 1 mL = 100 units on a U-100 syringe.
- Storage
- Lyophilized: refrigerate (2–8 °C), light-protected. Reconstituted in BAC water: refrigerate, use within 30 days.
TB-500 (Thymosin Beta-4) interactions
with BPC-157
CompatibleBPC-157 and TB-500 work via complementary pathways (nitric oxide vs actin binding) for synergistic soft-tissue and tendon repair.
Timing: Can be injected simultaneously in separate sites or the same syringe
with BPC-157
CompatibleThe classic healing combination. BPC-157 acts locally and systemically for tissue repair; TB-500 (Thymosin Beta-4) promotes cell migration and angiogenesis. Widely used for injury recovery.
Timing: Often combined as the 'healing stack'; administer daily or EOD
with GHK-Cu
CompatibleComplementary collagen and wound-healing peptides. GHK-Cu activates copper-dependent healing pathways; TB-500 promotes cell migration and angiogenesis. Excellent for skin, joint, and tissue repair.
Timing: Combine in the same injection or separate; both subcutaneous
with GHK-Cu
CompatibleComplementary healing and collagen synthesis peptides. TB-500 accelerates cell migration; GHK-Cu activates copper-dependent tissue repair enzymes. Synergistic for skin, tendons, and joints.
Timing: Combine in the same injection or administer separately; both subcutaneous
with GHK-Cu
CompatibleTB-500 accelerates cellular migration and angiogenesis while GHK-Cu drives collagen production and skin repair — complementary healing peptides with no known adverse interaction.
Timing: Can be combined in the same syringe or injected at the same time
with Testosterone
CompatibleTB-500 accelerates recovery from training stress. Higher training intensity on testosterone makes connective tissue repair more critical — a natural complement to any anabolic protocol.
Timing: TB-500 SubQ/IM 2x per week; independent of testosterone injection timing
with Thymosin Alpha-1
CompatibleThymosin Alpha-1 (immune modulation) and TB-500 (cellular repair/mobility) are a well-established recovery stack used in many longevity protocols. No known adverse interaction.
Timing: Inject both SubQ; independent timing
Commonly stacked with
Track TB-500 (Thymosin Beta-4) in Protokol.
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